AmniSure

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Definition

-  Rapid accurate qualitative test for in vitro detection of amniotic fluid in the vagina during pregnancy as an indicator of ruptured membranes

 

Features

-  Detects PAMG-1 protein as marker for amniontic fluid

-  Placental Alpha 1 Microglobulin (PAMG-1) is present in amniotic fluid

-  Normally low concentrations in blood & cervicovaginal secretions

-  Monoclonal antibody test using immunochromotography

-  More accurate test then ferning of amniotic fluid

-  Suitable for any gestational age

-  Small volume of blood, lubricant, sperm & urine does not interfere test

-  Does not require speculum exam

-  Often performed during speculum exam if ROM not evident

-  98.9% sensitivity, 100% specificity

 

Indications

-  PPROM

 

Equipment

-  Speculum

-  Sterile gloves

-  AmniSure test kit

-  Sterile dacron vaginal swab

-  Plastic vial w saline solvent

-  Amnisure test strip

-   Once foil open must be used by 6hrs

 

 

Procedure

-  +/- Speculum examination

-  Place vaginal swab into posterior fornix or pooled fluid if visualised for 1min

-  If pooled fluid is obviously amniotic fluid test not required

-  +/- inserted 2.5-3 inches into vagina wo spec

-  Place swab into vial w solvent for 1min+

-  Swirl swab in solvent

-  Dispose of swab

-  Place cap on solvent & shake

-  Dip AmniSure test strip into solvent

-  White end w arrows in first

-  Must be tested within 30min of collection

-  Leave test strip in solvent until x2 lines present or 10min

-  Do not interpret test after 10min

 

Results

-  Negative

-  1 distinct line: control line (no test line)

-  No amniotic fluid present, rupture very unlikely

-  Positive

-  2 lines: control + test line (brown/yellow)

-  Rupture of membranes possible

-  Invalid

-  No control line w or wo test line

 

 

 

 

References

 

Info + Pic (results, kit) www.amnisure.com/site/en/

Amnisure St Marys Hospital (Birthsuite), 11/08/07

AmniSure placental alpha microglobulin-1 rapid immunoassay versus standard diagnostic methods for detection of rupture of membranes, Am J Perinatol. 2005 Aug;22(6):317-20