Haemoglobin (Hb)

Last updated 05/12/16

Definition

Features

• Provides oxygen transport within Red Blood Cells (RBCs)
• Levels are assessed by various means
• Venepuncture and Full Blood Count (FBC)
• Haemoglobinometer
• Measurement typically by spectrophotometry
• Mostly automated
• RBCs broken down which releases Hb into solution
• Hb exposed to cyanide which forms cyanomethhaemoglobin (HbCN)
• Light shine though solution at 540 nanometers (absorption spectrum of HbCN) and absorption measures

Indications

• Anaemia
• Erythrocytosis

Results

• Adults
• Females 120-150 g/L
• Pregnancy
• Males 130-170 g/L
• Paediatric
• Reduced: anaemia
• Elevated: erythrocytosis

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References

1. RCPA - Hamoglobin