Viral Hepatitis Serology

Last updated 20.09.12

 

Description: Description: Icon-B-Index-Small

Index

 

Viral Serology

-  Hepatitis A Virus (HAV)

-  Hepatitis B Virus (HBV)

-  Hepatitis C Virus (HCV)

-  Hepatitis D Virus (HDV)

-  Hepatitis E Virus (HEV)

 

Procedure

-  Venepuncture: gold tube

 

 

Hepatitis A (HAV)

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Hepatitis A IgM Antibody (HepA IgM)

-  Acute infection (no chronic infections)

-  Small false positives with other infections

-  Usually detectable for 2-4 months post infection

-  May not be detectable at onset of disease

-  If highly suspicious check again in 7days

 

Hepatitis A IgG Antibody (HepA Ab Tot)

-  Past infection, immunisation or current infection

 

 

 

Hepatitis C (HCV)

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Hepatitis C Antibody (HepC Ab)

-  Does not DDx between acute, chronic or previous infection

-  50-70& +ve at onset of S/S

-  May take up to 3 months to be positive in disease

-  >90% +ve at 9 months

-  False positive common

 

Hep C Supplementary Antibody (HepC Supp Ab)

-  Second Ab test to confirm initial positive test

-  Different test to HepC Ab

 

HCV RNA PCR

-  Indicates actively replicating HCV

-  Levels vary with time

-  Absence in single test does not confirm resolution

 

Viral Load

 

Genotyping

-  PCR based

-  6 genotypes

-  Most important determinant of response to Rx

 

 

 

Hepatitis D (HDV)

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HBs Ag   

-  Requires HBV co-infection therefore +ve

 

HDV RNA

 

HDV IgM Ab

-  Acute or chronic infection

 

HDV IgG Ab

-  Chronic infection

 

 

 

Hepatitis E (HEV)

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HEV IgM Ab

-  Acute infection (no chronic infections)

 

HEV IgG Ab

-  Past infection

 

 

References

 

Western Diagnostic Group – Guide to Hepatitis Serology 2010