Kleihauer Test

Last updated 20.09.12

 

Index

 

Definition

-  Maternal blood test to determine volume of fetal Haemoglobin (HbF) transferred to Rh (D) negative maternal blood stream

 

Features

-  Determines dose of Anti-D required to neutralise HbF & prevent formation of antibodies & rhesus isoimmunisation

-  Rh (D) antibodies may cause Rhesus disease (anaemia, hydrops) in future Rh (D) positive fetuses

 

Indications

-  Fetomaternal haemorrhage

-  Sensitising events to determine dose of Anti-D required

-  Birth of Rh (D) postive baby to Rh (D) negative mother

-  Amniocentesis

-  APH

-  ECV

-  Unexpected/unexplained stillbirth

 

Procedure

-  Maternal venepuncture: pink tube (same as for cross match)

 

Results

-  Negative: indicates single Anti-D dose is sufficient

-  Positive will give indication of dose of Anti-D required to neutralise fetal RBCs

 

 

 

References

 

King Edward Memorial Hospital CPG: Obs & Gynae Guidelines 1.9.2: The Kleihauer Test, Feb 2011