Kleihauer Test

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Definition

-  Maternal blood test to determine volume of fotal Haemoglobin (HbF) transferred to Rh (D) negative maternal blood stream

 

Features

-  Determines dose of Anti-D required to neutralise HbF & prevent formation of antibodies

-  Rh (D) antibodies may cause Rh disease in future Rh (D) positive children

 

Indications

-  Fetomaternal haemorrhage

-  Sensitising events to determine dose of Anti-D

-  Birth of Rh (D) postive baby to Rh (D) negative mother

-  Amniocentesis

-  Antepartum haemorrhage

-  ECV

-  Unexpected/unexplained stillbirth

 

Procedure

-  Maternal venepuncture: pink tube (same as for cross match)

 

Results

-  Negative: indicates single Anti-D dose is sufficient

-  Positive will give indication of dose of Anti-D required to neutralise foetal RBCs

 

 

 

References

 

King Edward Memorial Hospital CPG: Obs & Gynae Guidelines 1.9.2: The Kleihauer Test, Feb 2011